%0 Journal Article
%A JIANG Cheng
%A LI Li
%A QIN Lin-Lin
%A ZHANG Xi
%T Cloning and Functional Analysis of <i>BpZFP</i>4 Promoter from Birch(<i>Betula platyphylla</i>)
%D 2019
%R 10.7525/j.issn.1673-5102.2019.06.015
%J Bulletin of Botanical Research
%P 917-926
%V 39
%N 6
%X The <i>BpZFP</i>4 plays significant roles in abiotic stress responses. To better understand the regulatory mechanisms of <i>BpZFP</i>4 gene expression, we cloned a 1360 bp long promoter fragment of <i>BpZFP</i>4 using genome walking technique. Computational analysis affirmed the existence of multiple abiotic stress responsive cis-elements as well as phytohormone, light, pathogen and wound responsive motifs. Deletion analysis of the <i>BpZFP</i>4 gene promoter in transgenic tobacco plants revealed the key regions and the possible cis-element regulating the <i>BpZFP</i>4 response to stresses such as drought, salt and ABA stress. This result may provide an important contribution for elucidating the regulation mechanisms of <i>BpZFP</i>4 expression in responding to abiotic stress and signalling molecule.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2019.06.015