%0 Journal Article
%A CUI Xin-Shuang
%A LI Xiao-Xue
%A WANG Fei
%A YANG Feng-Jian
%A YUAN De-Cheng
%T Extraction of <i>Perilla</i> Seed Oil by Aqueous Enzymatic Method
%D 2019
%R 10.7525/j.issn.1673-5102.2019.04.017
%J Bulletin of Botanical Research
%P 619-626
%V 39
%N 4
%X Central composite design(CCD)-response surface(RSM) was used to optimize the aqueous enzymatic extraction process of <i>Perilla</i> frutescens seed oil. On the basis of single factor experiment, we adopted the central composite design method to study the effects of enzymatic species, enzymatic hydrolysis temperature, pH, liquid-solid ratio, enzyme dosage and time interaction on the extraction rate of <i>Perilla</i> oil. The fitting equation was significant, the optimum conditions for the extraction of <i>Perilla</i> oil were as follows:pH is 9.5 in alkaline protease, the solid-liquid ratio(g) was 9.97:1, the amount of enzyme added was 2.75%, the temperature was 56.1℃, and the time was 5.25 h. Under this condition, the extraction rate of <i>Perilla</i> oil could reach 37.65%, which was very close to the theoretical value of 38.3%. The established model was true and reliable, and the optimum extraction process of <i>Perilla</i> oil was determined. The <i>Perilla</i> seed oil contains palmitic acid, stearic acid, oleic acid, linoleic acid and alpha-linolenic acid by gas chromatography, the relative content of alpha-linolenic acid in <i>Perilla</i> oil extracted by aqueous enzymatic method was 67.9%, and the physical and chemical indexes of relative solvent method and cold pressing method were the best.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2019.04.017