%0 Journal Article
%A FAN Er-Qin
%A FU Peng-Yue
%A LIU Cai-Xia
%A QU Guan-Zheng
%A YANG Chuan-Ping
%T Expression Pattern of <i>PagC</i>3<i>H</i>3 Gene in <i>Populus alba</i>&#215;<i>P.glandulosa</i>
%D 2019
%R 10.7525/j.issn.1673-5102.2019.04.006
%J Bulletin of Botanical Research
%P 521-528
%V 39
%N 4
%X As the main component of wood, lignin is usually polymerized from three monomers. During its biosynthesis, a total of ten enzyme families are involved in the conversion of phenylalanine to monomeric lignin, of which C3H is the key control enzymes in the hydroxylation process of <i>p</i>-coumaroyl CoA to caffeoyl CoA and in the formation of G/S monomer. Exploring the expression pattern of <i>PagC</i>3<i>H</i>3 gene is of great significance for further understanding of the function of this gene. In this study, the tissue-specific expression of <i>PagC</i>3<i>H</i>3 gene was analyzed by quantitative PCR; the promoter sequence of <i>PagC</i>3<i>H</i>3 with a length of 2 035 bp was cloned, and it was predicted to contain multiple cis-acting elements. At the same time, the promoter sequence of <i>PagC</i>3<i>H</i>3 was constructed to construct a plant expression vector pBI121-<i>PagC</i>3<i>H</i>3pro::<i>GUS</i> for transient transformation of <i>Arabidopsis thaliana</i>. The expression of <i>PagC</i>3<i>H</i>3 gene was higher in the roots, middle stem segments and basal stem segments of <i>Populus alba&#215;P. grandulos</i>; transient transformation of <i>A. thaliana</i> showed that GUS activity was stronger in hypocotyls and roots, suggesting that <i>PagC</i>3<i>H</i>3 gene plays a role in lignin synthesis.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2019.04.006