%0 Journal Article
%A FENG Yu
%A Han Mei-Ling
%A HOU Xiao-Qiang
%A LI Wen-Jing
%A LI Xiao-Xue
%A Sun Yan-Xiang
%A WANG Yong
%A XIANG Bei-Bei
%T Cloning, Bioinformatics and Expression of <em>SmSLS</em>2 Gene in <em>Swertia mussotii</em>
%D 2019
%R 10.7525/j.issn.1673-5102.2019.03.013
%J Bulletin of Botanical Research
%P 431-440
%V 39
%N 3
%X Secologanin synthase(SLS) is the key enzyme of secoiridoid pathway. We obtained the <em>SmSLS</em>2 gene full-length sequence according to the transcriptome of <em>Swertia mussotii</em>. We cloned the gene, analyzed the bioinformation of <em>SmSLS</em>2, constructed the vectors and performed the gene expression. The results showed that <em>SmSLS</em>2 cDNA complete sequence had a length of 1 566 bp(GenBank:MH535904), encoding 521 amino acid residues, and the pI of SmSLS2 was 8.92. Domain analysis results showed that SmSLS2 had one transmembrane domain, and the protein secondary and tertiary structures were analyzed and forecasted. The gDNA sequence of <em>SmSLS</em>2 was 2 576 bp, contained five exons and four introns. The SmSLS2 protein shared high identity with other SLS proteins of plants. Prokaryotic expression vector pET-28a-sumo-SmSLS2 was constructed and transformed into Escherichia coli BL21(DE3) for expression under 37℃, and induced by 0.5 mmol&middot;L<sup>-1</sup> IPTG. The SDS-PAGE results showed that the expressed proteins were consistent with the anticipated size. <em>SmSLS</em>2 gene was successfully cloned from <em>S.mussotii</em>, and it will provide a foundation for further functional research on SmSLS2 protein and increasing the product of iridoid compound by genetic engineering in <em>S.mussotii</em>.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2019.03.013