%0 Journal Article
%A HUANG Yan
%A WANG Xiao-Hong
%A YE Tian-Yi
%A ZHOU Xin
%T Key Technology of Micropropagation of <em>Osmanthus fragrans</em> var. <em>thunbergii</em>
%D 2019
%R 10.7525/j.issn.1673-5102.2019.03.005
%J Bulletin of Botanical Research
%P 358-364
%V 39
%N 3
%X We studied the effects of different rooting media, pre-treatment of seedling cultivation, transplanting matrix and leaf surface fertilization on the seedling cultivation of micro-propagation of <em>Osmanthus fragrans</em> to provide technical support for the rapid propagation of strong seedlings of target varieties, and the key technology of rapid propagation of <em>O.fragrans</em>. The optimal combination of rooting rate and rooting number of micro-propagated <em>O.fragrans</em> seedlings was A<sub>3</sub>B<sub>2</sub>C<sub>3</sub>, the rooting rate and average rooting number of B<sub>5</sub> basic medium+2 mg&middot;L<sup>-1</sup> NAA+0.10 mg&middot;L<sup>-1</sup> 6-BA were 91.11% and 3.83 pieces/plant, respectively. A<sub>3</sub>B<sub>2</sub>C<sub>3</sub> is the optimal combination for seedling refining, that is, when the nursery soil:perlite:humus=2:3:7 was used as the seedling refining matrix, the survival rate and growth rate of micropropagation seedlings of <em>O.fragrans</em> reached 82.2% and 42.2%, respectively.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2019.03.005