%0 Journal Article
%A CHEN Yuan
%A LIU Shi-Liang
%A PAN Yuan-Zhi
%A SHEN Li-Jia
%A ZHANG Lu
%T Embryonic Callus Induction and Plant Regeneration of Lilium leucanthum
%D 2019
%R 10.7525/j.issn.1673-5102.2019.03.003
%J Bulletin of Botanical Research
%P 338-346
%V 39
%N 3
%X With Lilium leucanthum Bakeri bulbs as explants, L9(34) orthogonal test was used to study the effects of different hormones and their concentrations on its callus induction, bulblet differentiation and rooting culture. Histological observation of embryogenic callus was carried out by paraffin sectioning technique. The optimal medium combinations for inducing callus of L.leucanthum under light and dark conditions were MS+TDZ 0.5 mg·L-1+NAA 1.0 mg·L-1+2, 4-D 0.05 mg·L-1 and MS+TDZ 0.5 mg·L-1+NAA 1.5 mg·L-1+2, 4-D 0.1 mg·L-1, with the induction rates of 62.690 and 59.423, respectively. The medium suitable for bulblet rooting was 1/2MS+NAA 0.2 mg·L-1+1 g AC. After 60-day culture, the seedlings were transferred to peat:vermiculite:perlite=1:1:1 in a medium and cultured in a light incubator for 35 days with the survival rate of 90%. Our experiment successfully established the regeneration system of squamous callus in L.leucanthum, which laid a foundation for the research of tissue culture transgenic seedling and the innovation of germplasm resources.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2019.03.003