%0 Journal Article
%A SHEN Bing-Qi
%A WANG Da-Wei
%A WANG Lian-Chun
%A ZHOU Fan
%T Characteristics of Microsatellite in <i>Camellia saluenensis</i> by High-throughput Sequencing
%D 2019
%R 10.7525/j.issn.1673-5102.2019.01.018
%J Bulletin of Botanical Research
%P 148-155
%V 39
%N 1
%X Leaves sample of <i>Camellia saluenensis</i> Stapf ex Bean was sequenced with high-throughput sequencing technology(Illumina Hiseq 2000). In total 140 996 transcripts of <i>C.saluenensis</i> were screened using MISA software. The 32 696 SSRs were identified, and the frequency of SSR was 23.2% and mean distribution density was dinucleotide 32.02 kB. The di-nucleotide repeats were the highest, followed by tri-nucleotide and mono-nucleotide repeat types. The tetra-nucleotide, penta-nucleotide and hexa-nucleotide were all less than 1%. Among the mononucleotide repeats, the A/T repeats motifs were the highest frequency(10.92%). AG/CT was the most frequent repeat motifs in di-nucleotide repeats(49.72%), followed by AT/AT and AC/GT. CG/CG were minimum. AAG/CTT repeats motifs was the highest in tri-nucleotide followed by ACC/GGT, ATC/ATG and AGG/CCT, while CCG/GGC, ACT/AGT and ACG/CGT were lowest(<1%). The repeat motifs were very few in tetra-nucleotide, petra-nucleotide and hexa-nucleotide and had no obvious differences. The microsatellite number in <i>C.saluenensis</i> decreased with the increased number of repeats and length of repeats.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2019.01.018