%0 Journal Article
%A CHEN Jing-Zhong
%A GUAN Rui-Ting
%A LIU Ji-Ming
%A TONG Bing-Li
%A WANG Min
%A WU Meng-Yao
%T Genetic Diversity of <i>Ampelocalamus luodianensis</i> in Different Microhabitats
%D 2019
%R 10.7525/j.issn.1673-5102.2019.01.009
%J Bulletin of Botanical Research
%P 69-77
%V 39
%N 1
%X By random amplified polymorphic DNA(RAPD) markers analysis, 5 kinds of karst microhabitats of <i>Ampelocalamus luodianensis</i> was conducted to study genetic diversity and genetic structure. Through 8 RAPD primers, 87 discernible DNA fragments in all weighted from 200-3 000 bp were cloned, and 68 in those were polymorphic loci, with percentage of polymorphic loci at the species level, <i>PPL</i>=78.16%, Nei's index <i>H</i>=0.291 3, and Shannon's Information Index <i>I</i>=0.431 0. The <i>A.luodianensis</i> grew on soil surface(TM) possessed the highest level of genetic diversity(<i>PPL</i>=47.13%, <i>H</i>=0.195 3 and <i>I</i>=0.283 4), while the <i>A.luodianensis</i> in stone crevice exhibited the lowest levels of genetic diversity(<i>PPL</i>=39.08%, <i>H</i>=0.147 4 and <i>I</i>=0.219 2). Coefficient of genetic differentiation (<i>G</i><sub>st</sub>) was 0.415 1(from the POPGENE32), and the gene flows(<i>N</i><sub>m</sub>) was 0.704 5. Among the 5 microhabitats, genetic identity varied from 0.777 3-0.999 4, genetic distance was different from 0.000 6-0.251 9, and the mean of them were 0.865 6-0.148 3 severally. Genetic diversity in niches at mean value was with <i>PPL</i> of 43.91%. Coefficient of genetic variation in niches was with <i>G</i><sub>st</sub> of 0.4054. The results indicated that differences of microhabitats increased the genetic diversity of <i>A.luodianensis</i>.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2019.01.009