%0 Journal Article
%A LI Wen-Jing
%A SUN Yan-Xiang
%T Cloning and Expression Analysis of Glutelin <i>GluC</i> Gene Promoter in Rice
%D 2018
%R 10.7525/j.issn.1673-5102.2018.06.016
%J Bulletin of Botanical Research
%P 921-930
%V 38
%N 6
%X Rice glutelin, which is expressed in endosperm exclusively, is ideal candidate for isolating promoters with endosperm specificity. We isolated the <i>GluC</i> 5'-flanking sequence pGluC. Analysis of pGluC sequence by PlantCARE and PLACE revealed it contained a series of endosperm specific elements, such as Skn-1 motif and ACGT-box. Thus, we constructed the pGluC and truncated pGluC into pGPTV-GUS vector and transferred the vectors into rice. We performed histochemical assay and GUS activity detection. The full-length and -1 911,-1 611, -1 311 bp promoters were mainly accumulated in endosperm. The -999, -451, -203, -102 bp promoters were mainly accumulated in endosperm, and less strongly in roots, stems and leaves, which changed their endosperm-specific expression pattern. Our findings would contribute to the selection of a suitable promoter for crop molecular improvement.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2018.06.016