%0 Journal Article
%A JIA Shan-Shan
%A WANG Yan-Yan
%A YU Fang
%T Ultrasound-Assisted Enhancement of Hair Induction Efficiency and Transgenic Hairy Root Construction of <i>Catharanthus roseus</i>
%D 2018
%R 10.7525/j.issn.1673-5102.2018.04.016
%J Bulletin of Botanical Research
%P 604-610
%V 38
%N 4
%X Based on the induction of hairy roots of <i>Catharanthus roseus</i> by different strains, different inducing sites, different media and different induction methods, we tried to change the infective reagents and the new ultrasound-assisted method. By comparing the hairy root induction efficiency and hair Morphological differences in root shape, we screened the optimal method and conditions for the induction of hairy roots of <i>C.roseus</i>, and established transgenic hairy roots. Finally, we used C58C1 as the inoculative strain. The leaves of the aseptic seedlings were used as explant materials, 1/2 MS medium was used as the induction medium for 2-day preculture time. The co-culture buffer was used to infect for 5 s. Ultrasound-assisted induction of optimal induction method succeeded in obtaining milky white, dense, non-directional and multi-branched hairy roots, and successfully constructing the hairy roots of <i>GUS</i> gene, which are related to metabolic regulation pathways later. This study laid the foundation on gene function and the synthesis of secondary metabolites of the <i>C.roseus</i>.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2018.04.016