%0 Journal Article
%A HU Xiao-Qing
%A LIU Xue-Mei
%A TIAN Jing
%A ZHANG Yong
%A ZHAO Tong
%A ZHU De-Cai
%T Cloning and Expression Analysis of Promoter of <i>CESA7</i> Gene in <i>Betula platyphylla</i>
%D 2018
%R 10.7525/j.issn.1673-5102.2018.04.009
%J Bulletin of Botanical Research
%P 551-558
%V 38
%N 4
%X A cellulosic synthase gene <i>Betula platyphylla</i>(<i>CESA</i>7) GenBank ID(EU591531) promoter was cloned and analyzed. By sequence analysis, the promoter was found to contain multiple cis-acting elements with different functions, including response elements, hormone-responsive elements, leaf morphology development elements, etc. It was speculated that this promoter plays a key role in the growth and development of birch. We cloned the promoter of <i>BpCESA</i>7 into a plant expression vector with a GUS reporter gene, named proBpCESA7-121-GUS, and invaded <i>B.platyphylla</i> and <i>Arabidopsis thaliana</i> by Agrobacterium-mediated method, and then observed that <i>BpCESA</i>7 gene was activated by GUS histochemical staining. Sub-organizational expression characteristics. Results GUS activity was detected in the roots, stems and leaves of <i>B.platyphylla</i> and Arabidopsis roots, leaves, sepals, and stamens, indicating that the promoter of <i>BpCESA</i>7 gene had promoter activity and was stained the most in the roots and leaves of birch, indicating <i>BpCESA</i>7 genes are highly expressed in the birch roots and leaves, and their tissue-specific expression is present.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2018.04.009