%0 Journal Article
%A HU Xiao-Qing
%A LIU Xue-Mei
%A TIAN Jing
%A XIN Qi-Qi
%A ZHAO Tong
%A ZHU De-Cai
%T Cloning and Expression Analysis of <i>APETALA2</i> Gene Promoter from <i>Betula platyphylla</i>
%D 2018
%R 10.7525/j.issn.1673-5102.2018.04.007
%J Bulletin of Botanical Research
%P 535-542
%V 38
%N 4
%X The <i>APETALA</i>2(<i>AP</i>2) transcription factor subfamily is ubiquitous in plants, and involved in the plant growth and development, stress response and signal transduction of various physiological and biochemical reactions. The full-length promoter sequence of <i>AP</i>2 gene was cloned from the genome of <i>Betula platyphylla</i> Suk. By bioinformatics analysis, this sequence has a large number of conserved components such as TATA-box and CAAT box, but also light responsive elements and hormone responsive elements, such as elements that respond to gibberellins, abscisic acid, and methyl jasmonate. The promoter of <i>AP</i>2 gene of <i>B.platyphylla</i> was cloned into pBI121-35S::GUS plant expression vector, named pBI121-proAP2::GUS, Agrobacterium-mediated infection of <i>B.platyphylla</i> and <i>A.thaliana</i>, and by GUS staining analysis, the GUS reporter gene driven by the promoter of <i>AP</i>2 gene is expressed throughout <i>A.thaliana</i> and is expressed in vegetative organs and female flower wings and in flower stalk of <i>B.platyphylla</i>, indicating that it has a priming activity that may participate in the development of this organ.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2018.04.007