%0 Journal Article
%A AN Yan-Huang
%A CHEN Lu
%A Wang Xin-Xia
%A YANG Ning
%A YANG Peng-Jun
%A ZHANG Xu-Qiang
%A ZHAO Min
%T Relationship Between Phospholipase Dδ and Nitric Oxide Responding to the Seed Germination in <i>Arabidopsis thaliana</i> under Drought Stress
%D 2018
%R 10.7525/j.issn.1673-5102.2018.04.006
%J Bulletin of Botanical Research
%P 526-534
%V 38
%N 4
%X With <i>Arabidopsis</i> wild-type(WT), nitric oxide synthase(NOS) deletion mutant(<i>noa</i>1), nitrate reductase(NR) deletion mutant(<i>nia</i>1 and <i>nia</i>2) and <i>PLDδ</i> deletion mutant(<i>pldδ</i>) of seedlings, we studied the relationship between <i>Arabidopsis thaliana PLDδ</i> and nitric oxide(NO) in response to the seed germination under drought stress. NO content, PLD and NR activity and gene relative expression were significantly increased under drought stress, while <i>pldδ</i> and <i>nia</i>2 are more sensitive than other mutants to drought stress. Sodium nitroprusside(SNP), as an exogenous NO donor, can improve the seed germination of WT, <i>nia</i>2 and <i>pldδ</i>. The exogenous phosphatidic acid(PA) can promote seed germination of WT and <i>pldδ</i> under drought stress except <i>nia</i>2. Besides, in compensation experiment, PA can promote the production of NO in WT and <i>pldδ</i> under drought stress, but not <i>nia</i>2 do. Therefore, it is speculated that PLDδ/PA located at the upstream of NO signal under drought stress, and the NO produced by NR2 is the main way for PLDδ/PA to promote <i>Arabidopsis</i> seed germination under drought stress.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2018.04.006