%0 Journal Article
%A CAO Ying
%A JIANG Hai-Gang
%A LI Xiang-Ning
%A LIU Yang
%A SHEN Yan-He
%A WANG Shu-Gang
%T Transcriptome-wide Identification of Lignin Gene Families of <em>Bambusa emeiensis</em> Shoots and Their Differential Expression
%D 2018
%R 10.7525/j.issn.1673-5102.2018.03.014
%J Bulletin of Botanical Research
%P 415-421
%V 38
%N 3
%X <em>Bambusa emeiensis</em> is a good fiber raw material, but the presence of lignin negatively affects bamboo pulp production. The 351 lignin biosynthesis-related unigenes of <em>B. emeiensis</em> were indentified through BLASTp combined with phylogenetic analysis, based on the transcriptome data of <em>B. emeiensis</em> shoots with 10 cm 150 cm height. Among them, the numbers of transcripts including 51 laccase(LAC), 374CL, 26PAL and 25CAD were found, and all these transcripts having more abundant in <em>B. emeiensis</em> than other reported bamboo species. RNA-seq and real-time quantitative PCR(RT-qPCR) analysis were used to investigate the differential expression of these lignin genes in bamboo shoots at the different elongation stages, and the relationship between lignin gene expression and lignin amount was further determined. The results revealed that with the elongation of shoots, the expression levels of 16 lignin biosynthesis genes, including <em>2 PAL, 5 CCR, 34CL, 2CAD</em> and <em>4LAC</em>, were up-regulated, indicating their roles in developmental lignin accumulation of <em>B. emeiensis</em>.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2018.03.014