%0 Journal Article
%A CHEN Chu
%A LIU Yi
%A LONG Min-Hui
%A SHU Liang-Zuo
%A SONG Yun-Xian
%A WU Xiao-Min
%A ZHANG Qiang
%T Preliminary Study on the Effects of Two Different Nitrogen Forms on Flavonoids Accumulation and Related Molecular Mechanism in Medicinal Plant <em>Tetrastigma hemsleyanum</em>
%D 2018
%R 10.7525/j.issn.1673-5102.2018.03.008
%J Bulletin of Botanical Research
%P 367-376
%V 38
%N 3
%X The effects of two different nitrogen(N) forms(nitrate-N and ammonium-N) on the accumulation of flavonoids(procyanidin B1, rutin, kaempferol-3-O-rutinoside, quercetin, isoquercitrin and kaempferol) in the endangered medicinal plant <em>Tetrastigma hemsleyanum</em>(from Zhejiang Province) were examined through potted experiment under shady condition(30% natural light), and its influence mechanism was also discussed. The flavonoids content was relatively higher in the root and leaf of <em>T. hemsleyanum</em> under nitrate-N supply than ammonium-N supply. The levels of transcription and expression of phenylalanine ammonia-lyase(PAL) and chalcone synthase(CHS) which are related to flavonoids synthesis were relatively higher in the root and leaf of <em>T. hemsleyanum</em> under nitrate-N supply than ammonium-N supply. Besides, the enzyme activities of PAL and CHS were also higher in the root and leaf of <em>T. hemsleyanum</em> under nitrate-N supply than ammonium-N supply. The PAL-specific inhibitor(AOA) could significantly inhibit the enzyme activity of PAL(<em>P</em>&lt;0.05) and lead to a very significant decrease in flavonoids content(<em>P</em>&lt;0.01). In conclusion, nitrate-N supply is more beneficial to flavonoids synthesis in <em>T. hemsleyanum</em>, which mainly enhances the flavonoids synthesis by increasing the gene expression and activity of key enzymes in PAL metabolic pathway.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2018.03.008