%0 Journal Article
%A JIANG Ji-Hong
%A LI Dan
%A MENG Lai-Sheng
%A XU Meng-Ke
%T <em>Arabidopsis thaliana</em> Transcription Factor Ethylene-insensitive3(EIN3) Inhibits the Synthesis of Anthocyanins
%D 2018
%R 10.7525/j.issn.1673-5102.2018.01.018
%J Bulletin of Botanical Research
%P 148-154
%V 38
%N 1
%X Ethylene-insensitive3(EIN3) and EIN3-like1(EIL1) proteins are important nuclear transcription factors in ethylene signal transduction pathways in higher plants. In plants, anthocyanins are one of the water-soluble natural pigments, and they play an important role in the growth and development of plants. We used the <em>Arabidopsis thaliana</em> double mutant <em>ein</em>3-1<em>eil</em>1-3 as the research material. Firstly, we identified the mutants of <em>ein</em>3-1<em>eil</em>1-3 and <em>ein</em>3-1 by RT-PCR. The direct observation indicated that the seeds and leaves of the <em>ein</em>3-1<em>eil</em>1-3 were purple. By ultraviolet spectrophotometer analysis, the anthocyanin accumulation in <em>ein</em>3-1<em>eil</em>1-3 mutant was significantly higher than that in the <em>ein</em>3-1 mutant and wild-type. <em>GUS</em> staining showed that <em>EIN3</em> was mainly expressed in flowers, stigma, mature pollen, seed embryo and siliques, which was consistent with that anthocyanin accumulation of seeds and leaves was high in mutant <em>ein</em>3-1<em>eil</em>1-3. Thus, <em>A.thaliana</em> transcription factor EIN3 as well as EIL1 is involved in the suppression of the anthocyanin synthesis.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2018.01.018