%0 Journal Article
%A DU Zhong-Yu
%A DUAN Yi-Zhong
%A KANG Fu-Ren
%A WANG Jian-Wu
%T SNP Sites Developed by Specific Length Amplification Fragment Sequencing(SLAF-seq) and Genetic Analysis in <em>Ammopitanthus mongolicus</em>
%D 2018
%R 10.7525/j.issn.1673-5102.2018.01.017
%J Bulletin of Botanical Research
%P 141-147
%V 38
%N 1
%X Ten site of <em>Ammopiptanthus mongolicus</em> distributed in Inner Mongolia, Ningxia and Gansu Province were used as experiment materials for sequencing by specific-locus amplified fragment sequencing(SLAF-seq). The scheme of the experiment was designed based on bio-informatics technology. Taking Glycine max as the reference genome, specific size of DNA were chosen to construct the SLAF-seq library. After high-throughput sequencing, a great amount of sequences were obtained and used to obtain the polymorphism SLAF tags by software alignment, then found the distribution of specific SNP sites. In total, we obtained 374 265 SLAF tags, including 56 295 polymorphic SLAF ta