%0 Journal Article
%A WANG Chao
%A WANG Yan-Min
%A WANG Yu-Cheng
%A ZHANG Chun-Rui
%A ZHANG Yu
%A ZHANG Yue
%T Cloning and Expression Analysis of S-Adenosine Methionine Decarboxylase(<em>ThSAMDC</em>) Gene from <em>Tamarix ramosissima</em>
%D 2018
%R 10.7525/j.issn.1673-5102.2018.01.016
%J Bulletin of Botanical Research
%P 132-140
%V 38
%N 1
%X S-adenosyl-L-methionine decarboxylase(<em>SAMDC</em>) is a rate-limiting enzyme that regulates the physiological and biochemical processes of plants by participating in the metabolic pathway of polyamines. The cDNA of S-Adenosine Methionine Decarboxylase gene(named <em>ThSAMDC</em>) was isolated and cloned by analyzing the transcriptome data of <em>Tamarix hispida</em>. The full-length of <em>ThSAMDC</em> is 2 085 bp, with three open reading frames, tiny ORF(tORF), upstream ORF(uORF) and main ORF(mORF). The mORF was 1 107 bp encoding 369 amino acids. The relative molecular weight and isoelectric points(PI) of the putative protein were 40.34 kD and 4.72. <em>ThSAMDC</em>-encoded protein has a number of strong hydrophilic regions, no obvious transmembrane region. Compared to the amino acid multiple sequence alignment of several other species, ThSAMDC includes two highly conserved domains: the proenzyme cleavage site(LSESSLF) and the PEST domain(TIHVTPEDGFSYAS) associated with the rapid degradation of the protein. Phylogenetic tree analysis shows that <em>ThSAMDC</em> has higher sequence similarity of 77% identities to the <em>Spinach</em>(SoSAMDC). Quantitative real-time PCR assay revealed that the mRNA level of <em>ThSAMDC</em> was significantly up-regulated under NaCl, PEG, ABA and CdCl<sub>2</sub> treatments in <em>T.hispida</em>, suggesting that <em>ThSAMDC</em> may play an important role in drought resistance, salt tolerance and other stresses.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2018.01.016