%0 Journal Article
%A FU Yu-Jie
%A JIAO Jiao
%A KOU Ping
%A SU Wen
%A WANG Peng
%A YANG Ming-Fei
%A YANG Yu-Chun
%A ZHANG Jing
%A ZHANG Sun-Dong
%T The Optimized Proportion of Combination Pinoleic Acid/L-Carnitine Decrease Lipid in HepG2
%D 2017
%R 10.7525/j.issn.1673-5102.2017.06.018
%J Bulletin of Botanical Research
%P 941-946
%V 37
%N 6
%X As the single active ingredient is poor and prone to drug resistance on effects of lose weight and lipid-lowering, so the study focuses on screening and optimizing the optimal concentration ratio of pinoleic acid/L-carnitine to reduce the lipid of HepG2 cell, which provides the basic data for the development of pinoleic acid lipid-lowering products. Oleic acid induced HepG2 cell adipogenesis, which was used to simulate the model of nonalcoholic fatty liver in vitro. The optimal concentration in combination of pinoleic acid/L-Carnitine decrease lipid was determinated by oil red O staining. The results show that the optimum concentration of oleic acid-induced HepG2 cell steatosis is 0.5 mmol&middot;L<sup>-1</sup>.The optimal lipolysis concentration of pinoleic acid is 6.25 &mu;mol&middot;L<sup>-1</sup>. The optimal lipid metabolism concentration of L-Carnitine is 250 &mu;mol&middot;L<sup>-1</sup>. The best lipid-lowering ratio of pinoleic acid/L-carnitine(PLA/LC) is 1:40(&mu;mol&middot;L<sup>-1</sup>/&mu;mol&middot;L<sup>-1</sup>). The characteristics of pinoleic acid and L-Carnitine complexes decreased the doses and improved synergistic effect, Therefore, Combination of pinoleic acid/L-Carnitine has a potential prospectin in the development of lipid-lowering diet products.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2017.06.018