%0 Journal Article
%A CHENG Fang-Yun
%A XU Li
%A ZHONG Yuan
%T Study on Rapid Seedling-raising Technology of Tree Peony Embryo Culture
%D 2017
%R 10.7525/j.issn.1673-5102.2017.05.008
%J Bulletin of Botanical Research
%P 690-699
%V 37
%N 5
%X This study used Paeonia ostii ‘Fengdan’ and Paeonia rockii as explants, established efficient method of Seedling-raising of tree peony embryo culture through the study of hormones, light conditions, medium ingredients and activated carbon(AC) on the in vitro embryo culture. The main result:In vitro embryos radicle germination in 10 d, grow true leaves in 20 d, root system developed in 25 d, transplanting in 40 d, after 3 months the seedlings grow well in the medium of improved MS(calcium doubled)+0.2 g·L-1AC+0.5 mg·L-1GA+0.5 mg·L-1BA. Suitable medium for ‘Fengdan’:improved MS(calcium doubled,macroelement doubled)+0.6 g·L-1AC+1.0 mg·L-1GA; medium for P.rockii:improved MS(calcium doubled,macroelement doubled)+0.6 g·L-1 AC+0.5 mg·L-1GA; The seedling rate of ‘Fengdan’ was 63.88%, the survival rate was 66.34%, and the rate of seedling of P.rockii was 93.75%, and survival rates vary among breeds, with a maximum of 100%.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2017.05.008