%0 Journal Article
%A BAI Xiao-Ming
%A LI Shu-Juan
%A SONG Yue
%A ZHANG Han-Guo
%T Response Surface Optimization of Culture Conditions for the Formation of Early Embryos in <i>Larix olgensis</i>
%D 2017
%R 10.7525/j.issn.1673-5102.2017.04.018
%J Bulletin of Botanical Research
%P 613-620
%V 37
%N 4
%X With the larch embryogenic callus, through the Design Box-Behnken design test, we established a response surface model based on the somatic embryogenesis. The culture conditions of somatic embryo early maturation was optimized in <i>Larix olgensis</i>. The relative ion concentration(<i>P</i>=0.007), the inositol concentration(<i>P</i>=0.000), the culture period(<i>P</i>=0.000) and the interaction between inositol content and culture period(<i>P</i>=0.007) effect on the number of somatic embryos of <i>L.olgensis</i> were extremely significant. Cultivation of Larix somatic embryo maturation was 337.04 ind&#183;g<sup>-1</sup> at the early stage of the most optimum conditions for relative ion content of 26.767 7% BM basic culture medium, inositol 10.454 5 g&#183;L<sup>-1</sup>, culture time 12.656 6 d, and the number of somatic embryos average. In order to facilitate the practical operation, the culture condition was changed to the relative ion concentration of 25% BM, inositol 10 g&#183;L<sup>-1</sup>, incubation period of 13 d, then the average number of somatic embryos was 336.46 ind&#183;g<sup>-1</sup>, that was consistent with the predicted value 336.29 ind&#183;g<sup>-1</sup>. Compared with previous test, the quantity and quality of <i>L.olgensis</i> somatic embryos were increased significantly.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2017.04.018