%0 Journal Article
%A LI Hai-Quan
%A LI Jian-Yue*
%A LI Yuan
%A XIAO Gang
%T Producing IGF-1 in Transgenic Tobacco
%D 2011
%R 10.7525/j.issn.1673-5102.2011.03.015
%J Bulletin of Botanical Research
%P 336-340
%V 31
%N 3
%X In this research, a new expression vector of pCAMBIA1301-35S promoter-<em>igf</em>-1-nos, which included the gene of hIGF-1, was constructed. The gene is promoted by CaMV 35S promoter which can be expressed in tobacco in high performance. The expression vector of IGF-1 cDNA introduced by 35S promoter was transformed into LBA4404 (<em>Agrobacterium tumefacients</em>), and then infected tobacco through leaf-disc infection. 17 transgenic tobacco plants were gained after hygromycin selection, GUS test and PCR test of DNA. It shows IGF-1 gene can be transcribed into normal mRNA in transgenic tobacco by RT-PCR test. In this experiment, the expression of IGF-1 in tobacco lays the foundation for producing IGF-1 in tobacco and other dicotyledon bio-reactors as well.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2011.03.015