%0 Journal Article
%A HU Ou
%A HUANG Qing-Yun
%A HUANG Yi-Jin
%A LIN Chun-Song
%A XU Su-Xia
%T Comparison between Betalain and Flavonol Accumulation in Tricolor <em>Bougainvillea peruviana</em> &lsquo;Thimma&rsquo; Based on Transcriptome
%D 2017
%R 10.7525/j.issn.1673-5102.2017.02.013
%J Bulletin of Botanical Research
%P 249-258
%V 37
%N 2
%X <em>Bougainvillea peruviana</em> &lsquo;Thimma&rsquo;, accumulating betalains, not anthocyanins occurring in majority of plants. The cultivar was characterized with either white, magenta, and white-magenta(variegated) bracts in the same branches. We first used magenta(Yp), bicolor(Ym) and white(Yw) open inflorescences for transcriptome analysis by sequencing. Candidate genes involving in pigment metablisms were validated by real-time PCR. We obtained 73,325 genes with average length of 616 bp. Out of DEGs, 327 candidate genes of betalain-biosynthesis, 308 flavonoid-biosynthesis genes and 466 anthocyanin-accumulation genes were detected. Eight candidate pigment-related genes were verified, four were responsible for betalain production(PPO, CYP76AD1, cDOPA-5-GT, DODA), and four responsible for flavonoids production(FLS, DFR, LDOX, 3-GT). Expression strength of four betalain production genes was well consistent with betalain-accumulation in Yp, Ym and Yw. Full later three anthocyanidin-producing genes(DFR, LDOX, 3-GT) were first verified and the expression varied in three samples. Expression of all eight candidate genes was well consistent with RNA-sequencing data. <em>B.peruviana</em> could provide a good tool to study physiological, phytochemical and molecular mechanisms in <em>Bougainvillea</em>, effectively eliminating the interference of some factors.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2017.02.013