%0 Journal Article
%A HU Lu-Jie
%A YANG Chao-Dong
%A ZHANG Xia
%A ZHOU Cun-Yu
%T Comparative Study on Staining Methods and Techniques of Cell Wall Histochemistry
%D 2017
%R 10.7525/j.issn.1673-5102.2017.01.019
%J Bulletin of Botanical Research
%P 147-154
%V 37
%N 1
%X We carried out the comparative studies on several methods and techniques for cell wall histochemistry under light and epifluorescence microscope. The results showed that:(1) To study Casparian bands using staining methods of sulphuric acid digestion and berberine hemisulfate-aniline, the requirements of material collecting time and part were high, therefore, it was recommended to use the two methods to mutual confirmation; (2) To study suberin lamellae using Sudan red 7B staining, and none staining and berberine hemisulfate-aniline blue staining under blue excited, none staining was more sensitive than Sudan red 7B, but Sudan red 7B staining is convenient under light microscope; (3) To study lignified walls, berberine hemisulfate-aniline staining was more sensitive than phloroglucinol-HCl; (4) Using TBO fast wall staining to replace Sudan Ⅲ/Ⅳ conventional staining, the wall borders and layers were clearer.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2017.01.019