%0 Journal Article
%A CAO Ying
%A HU Shang-Lian
%A LI Bang-Yong
%A LI Hui-Ping
%T Cloning and Expression Analysis of Two NAC Transcription Factors in <em>Bambusa emeiensis</em>
%D 2016
%R 10.7525/j.issn.1673-5102.2016.06.013
%J Bulletin of Botanical Research
%P 895-901
%V 36
%N 6
%X Based on the transcriptome database of <em>Bambusa emeiensis</em> and genome information of <em>Phyllostachys heterocycla</em>, the two NAC genes were cloned by homology cloning technology from the shoots of <em>B.emeiensis</em>, named as <em>BeNAC</em>1(GenBank:KU550706) and <em>BeNAC</em>2(GenBank:KU821586), encoding 313 and 389 amino acids, respectively. By protein function prediction and conserved domain multiple alignment, both of <em>BeNAC</em>1 and <em>BeNAC</em>2 contained NAM conserved region. By protein structure prediction, the putatived three-dimensional structure of <em>BeNAC</em>1 and <em>BeNAC</em>2 protein was similar to a rice protein with known crystal structure. By subcellular localization prediction analysis, BeNAC1 was mainly concentrated in the cytoplasm, and BeNAC2 was mainly concentrated in the nucleus. Tissue expression pattern analysis showed that <em>BeNAC</em>1 and <em>BeNAC</em>2 displayed similar tissue-specific, both having the highest expression on stems, whereas in addition to the bamboo shoots, the expression of <em>BeNAC</em>1 in each tissue tested was higher than that of <em>BeNAC</em>2.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2016.06.013