%0 Journal Article
%A CHEN Shi-Long
%A GAO Qing-Bo
%A TIAN Zun-Zhe
%A ZHANG Fa-Qi
%T Characteristics of SSR and SNP in <em>Gentiana veitchiorum</em> in Qinghai-Tibetan Plateau,by High-throughput Sequencing
%D 2016
%R 10.7525/j.issn.1673-5102.2016.05.016
%J Bulletin of Botanical Research
%P 747-752
%V 36
%N 5
%X We used the next generation sequencing(NGS) to capture the SSR and SNP marker in <em>Gentiana veitchiorum</em>, sampled in Zeku, Qinghai Province. A total of 8588 simple sequence repeats were generated through lumina HiSeq<sup>TM</sup> 2500. The dinucletide repeats were the highest(54.7%), followed by the trinucleotide repeats(41.3%). A proportion of tetranucletide, pentanucleotide and hexanucleotide repeats was less, which is 4% in total. In dinucleotide repeats, AT/TA repeats were the dominated ones, 9.85% and 9.5%, respectively. The length of nucleotide repeat and number of repeat were negative correlation, while there was a positive correlation between the total length of SSR sequences and the length of nucleotide repeat. A total of 253 789 and 249 417 SNPs were indenfied in GP-F and GP-L of <em>G.veitchiorum</em>. The proportion of the SNPs located in the noncoding region were 51.29% and 51.96%, respectively. The proportion of synonymous transition(48.63% and 47.96%) in coding region was significantly higher than that of nonsynonymous transition(0.08% and 0.08%), which might be caused by relatively conservative domains of functional genes.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2016.05.016