%0 Journal Article %A HU Feng %A HUANG Lie-Jian %A WANG Hong %T Bud Inducing from 16-year Elite Trees Explants of Three Acacia Species %D 2016 %R 10.7525/j.issn.1673-5102.2016.05.014 %J Bulletin of Botanical Research %P 730-738 %V 36 %N 5 %X The explants from 16-year elite trees of Acacia mangium, A.auriculiformis and A.mangium×A.auriculiformis were used to establish the efficient bud inducing technique, by studying the sterilization methods, position, gathered time and type of explants, the effects of 6-BA, sucrose, and basal medium. A.mangium was disinfectant sensitive, treatment with mercuric chloride 9 min and ethanol 15 s was the optimal, but longer time were need for A.auriculiformis and A.mangium×A.auriculiformis, treatment with mercuric chloride 18 min, ethanol 15 s and mercuric chloride 15 min, and ethanol 30 s, respectively. These three Acacia species shared common optimal position, gathered season and type:3-5^th node of shoot as explants were optimal position. It was the optimal season to gather explants in August; Type 2 explant germinated from shoots of cutting orchard (established by cutting the shoots of mature elite) was the optimal type; The optimal bud inducing medium were modify MS+6-BA 0.5 mg·L^-1+sucrose 30 g·L^-1 for A.mangium, MS+6-BA 1.0 mg·L^-1+sucrose 40 g·L^-1 for A.auriculiformis, MS+ sucrose 20 g·L^-1 for A.mangium×A.auriculiformis, with the highest germination rate of 93.33%, 92.00% and 97.33%, respectively. %U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2016.05.014