%0 Journal Article
%A LAN Xing-Guo
%A LI Han
%A LI Xiao-Yu
%A LI Yu-Hua
%A LI Zhi-Long
%T Selection of Reference Genes for Real-time Fluorescence Quantitative PCR in Different Tissues and Stigma Development from Ornamental Kale
%D 2016
%R 10.7525/j.issn.1673-5102.2016.04.012
%J Bulletin of Botanical Research
%P 565-572
%V 36
%N 4
%X We used real-time quantitative polymerase chain reaction to test the expression of Actin, cysteine proteinase inhibitor 6(Cpi6), elongation factor 1-beta(EF-1β), glyceraldehyde-3-phosphate dehydrogenase(GAPDH), tubulin alpha-3(Tub-α3), tubulin alpha-6(Tub-α6) and ubiquitin conjugating enzyme7(Ubc7), and then identified the most suitable reference genes in a given set of tissues and different developmental stigmas of ornamental kale(Brassica oleracea var. acephala) S13-bS13-b homozygotes by using the geNorm and BestKeeper software programs. By a geNorm analysis, Tub-α6 and EF-1β were the most suitable reference genes among the given set of tissues, and Actin and Ubc7 were the most stable genes during stigma development. By a BestKeeper analysis, Ubc7 and Tub-α6 were the most suitable reference genes among the given set of tissues, and EF-1β and Tub-α6 were the most stable genes during stigma development. Tub-α6 and Actin were the most suitable reference genes among the given set of tissues and during stigma development, respectively. Furthermore, the expression of SLG normalized with Tub-α6 or Actin showed that SLG was predominantly expressed in stigma among the given set of tissues and reached its highest level at approaching anthesis during stigma development.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2016.04.012