%0 Journal Article
%A KONG Jin
%A NIE Ming-zhu
%A SHI Fu-chen
%A TANG Zhong-hua
%A YU Jing-hua
%A ZU Yuan-gang
%T THE STUDY ON AGROBACTERIUM TUMIFACIENS-MEDIATED TRANSFORMATION FOR WHITE BIRCH(<em>BETULA PLATYPHYLLA</em>) FLASK CONTAINING GUS
%D 2002
%R 
%J Bulletin of Botanical Research
%P 247-251
%V 22
%N 2
%X Agrobacterium tumifaciens-mediated transformation for leaf disc of <em>Betula platyphylla</em> containing GUS was investigated and factors on Agrobacterium tumefaciens-mediated gene transfer is also discussed in detail. The optimal concentration of kanamycin to strain fungus is 50mg/l and of carbenicillin and cefotaxime is 500mg/l. Regeneration ratio of adventitious buds will improve 3-fold by adding of 100&mu;M AS to regeneration medium. The optimal pre-cultivation period for leaf disc transformation is 3~4 days, the ideal time for leaf disc dipping in Agrobacterium is 10~20 minutes, and the optimal co-cultivation is 4 days. Transformed plants of white birch were obtained under above conditions and the transformation rate is 2.8%.
%U https://bbr.nefu.edu.cn/EN/abstract/article_2711.shtml