%0 Journal Article
%A CHU Yan-Guang
%A HOU Ying-Jie
%A LIU Gui-Feng
%A WANG Yu-Cheng
%T Construction and analysis of <em>Tamarix hispida</em> suppression subtractive hybridization library under drought stress
%D 2005
%R 10.7525/j.issn.1673-5102.2005.01.021
%J Bulletin of Botanical Research
%P 69-73
%V 25
%N 1
%X Using cDNA from <em>Tamarix hispida</em> roots treated with drought stress as tester and cDNA from <em>Tamarix hispida</em> roots in normal growth as driver, suppression subtractive hybridization (SSH) was employed to construct cDNA subtracted library. In the library, the rate of recombination was 95%, the size of inserts was 250~600 bp. 17 drought stress-associated genes were obtained by DNA sequencing the positive clones picked randomly, such asMn-SOD myb-related protein zinc finger protein, etc. These genes included osmotic regulator signal component regulatory protein and antioxidant enzyme. GenBank had accepted all the sequences. The research had established a basis for cloning stress-resistance genes and further studying genes expression in <em>Tamarix hispida</em> roots under drought stress.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2005.01.021