%0 Journal Article
%A PENG Yong-Kang
%A WANG Zhen-Ying
%A ZHENG Jian-Yu
%T Cloning and sequencing analysis on the cDNA fragment SI<sub>800</sub> separated from rye which related with salt tolerance
%D 2005
%R 10.7525/j.issn.1673-5102.2005.04.011
%J Bulletin of Botanical Research
%P 427-431
%V 25
%N 4
%X SI<sub>800</sub> (Salt Induced(800 bp), SI<sub>800</sub>) was a cDNA fragment, which separated from rye leaves by DDRT-PCR.In this study, we purified SI<sub>800</sub> by low temperature agarose electrophoresis and linked to pGEM-T vector then transformed to <em>Escherichia coli</em>(JM 109).30 positive clones were obtained.2 positive clones were selected randomly after test by digestion, PCR and sequenced.The result indicated that SI<sub>800</sub> was composed of(735 bp).DNA sequence analysis showed(68%) homologue to uxaB gene for altronate oxidoreductase in GenBank.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2005.04.011