%0 Journal Article
%A FU Yu-Jie
%A HOU Chun-Lian
%A LIU Xiao-Na
%A SHI Xiao-Guang
%A ZHAO Wen-Hao
%A ZU Yuan-Gang
%T Determination of the Glycyrrhizic acid content from <em>Glycyrrhiza uralensis</em> by HPLC
%D 2005
%R 10.7525/j.issn.1673-5102.2005.02.021
%J Bulletin of Botanical Research
%P 210-212
%V 25
%N 2
%X Extracted Glycyrrhizic acid (GA) from <em>Glycyrrhiza uralensis</em> Fisch by Ultrasonic extraction method, the conditions with higher GA were 0.3% ammonia solution as solvent, ratio of liquid to solid as 50:1(mL/g), extraction time five hours. The HPLC analysis conditions were as follows, chromatographic column ODS, detecting wavelength 254 nm, room temperature, mobile phase CH 3OH/3% HOAc(V/V) which ratio was 75/25, flowing rate 1 mL/min,injected sample volume 10 <em>&mu;</em>L. The result of percent recovery is 100.20%, the relative standard deviation is 1.77%. Ultrasonic extraction-HPLC is a fast detecting GA method with higher accuracy from <em>Glycyrrhiza uralensis</em>.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2005.02.021