%0 Journal Article
%A XU Jin-Bang
%A ZHANG Jun-Yi*
%T Analysis of <em>Liriope spicata</em> and Its Related Species Based on DNA Barcoding Technique
%D 2012
%R 10.7525/j.issn.1673-5102.2012.04.013
%J Bulletin of Botanical Research
%P 452-457
%V 32
%N 4
%X To discuss the potential of cpDNA <em>trn</em>L-F and <em>psb</em>A-<em>trn</em>H as DNA barcode of <em>Liriope spicata</em>(Thunberg) Loureiro and its related species, cpDNA <em>trn</em>L-F and <em>psb</em>A-<em>trn</em>H sequences were analysed. These two regions were respectively amplified by PCR and then purified and sequenced successfully. The results showed that the lengths of <em>trn</em>L-F sequences in 18 plant samples belonging to 9 species of liriopogons varied from 355 to 356 bp. There was no difference in the <em>trn</em>L-F of various species of liriope, but some specifically variable sites were found in this fragment, which led to the identification between <em>Ophiopogon japonicus</em>(Thunb.) Ker-Gawl and Ophiopogon bodinieri Levl. The length of <em>psb</em>A-<em>trn</em>H region was 543-544 bp, there were only some identification sites in <em>O.japonicus</em>(Thunb.) Ker-Gawl. Because of the low rate of evolution and the high conservation, these two cpDNA spacer regions could be only appropriate as a DNA barcode to identify liriopogons at generic level, but there is a degree of limit at lower taxonomic level.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2012.04.013