%0 Journal Article
%A FAN Hai*
%A GAO Bin
%A ZHANG Hai-Yan
%T Expression,Purification and Immunoreactivity of Hygromycin-B-Phosphotransferase
%D 2011
%R 10.7525/j.issn.1673-5102.2011.01.010
%J Bulletin of Botanical Research
%P 56-60
%V 31
%N 1
%X The prokaryotic expression plasmid of hygromycin-B-phosphotransferase(HPT) was constructed. The hpt gene was cloned by PCR. It was digested by <em>Sal</em>Ⅰ/<em>Not</em>Ⅰand subcloned into expression vector pET-28b(+). pET-28b-<em>hpt</em> was transferred into E. coli Rosset; fusion protein was induced by isopropyl-&beta;-D-thiogalactopyranoside (IPTG) and analyzed by SDS-PAGE. The most products existed in an inclusion body form. The HPT protein purified by Ni<sup>2+</sup>-NTA column was used to immunize New Zealand rabbits. The HPT polyclonal antibodies reveal high sensitivity and specificity.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2011.01.010