%0 Journal Article
%A LI Ying
%A LIU Dian-Kun
%A QU Guan-Zheng
%A SI Dong-Jing
%A YAN Dong
%A ZHAO Xi-Yang
%A ZHENG Mi
%T Optional Protein Extracting Methods of Poplar Leaves for Two-dimensional Gel Electrophoresis
%D 2016
%R 10.7525/j.issn.1673-5102.2016.03.022
%J Bulletin of Botanical Research
%P 469-475
%V 36
%N 3
%X In order to obtain the two-dimensional gel electrophoresis images with better separation and definition of poplar leaves, the <em>Populus</em> X, <em>P.trichocarpa</em> Torr. &amp; Gray and <em>P.alba&times;P.glandulosa</em> were selected to study the optional extracting methods of poplar leaves for two-dimensional gel electrophoresis by using TCA/acetone precipitation, Tris/saturated phenol and Tris/TCA method, respectively. The proteins of poplar leaves were separated by SDS-polyacrylamide gel electrophoresis and two-dimensional gel electrophoresis respectively and the images of two-dimensional gel electrophoresis were analyzed by Image Master 2D Platinum 6.0. Tris/TCA method was more appropriate for the protein extraction of poplar leaves for two-dimensional gel electrophoresis. The average protein content of the fresh sample extracted by Tris/TCA method was 949.46 &mu;g&middot;g<sup>-1</sup> and the average quantity of protein spots was 567 in the two-dimensional gel electrophoresis images which had better separation and definition.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2016.03.022