%0 Journal Article
%A DAI Li-Juan
%A LIU Cai-Xia
%A QU Guan-Zheng*
%A ZANG Li-Na
%A ZHENG Tang-Chun
%T Identification of Protein Interactions between Flowering Repressors BpFLC and BpSVP from <em>Betula platyphylla</em>
%D 2015
%R 10.7525/j.issn.1673-5102.2015.06.013
%J Bulletin of Botanical Research
%P 866-872
%V 35
%N 6
%X The experiment was conducted to study the mechanism of interaction between SVP and FLC in <em>Betula platyphylla</em>. The truncated genes of <em>BpFLC</em>1-6 and <em>BpSVP</em>1-6 were, respectively, cloned from yeast recombination plasmids pGBKT7-<em>BpFLC</em> and pGBKT7-<em>BpSVP</em>. pGBKT7-<em>BpFLC</em>1-6&times;pGADT7-<em>BpSVP</em> and pGBKT7-<em>BpFLC</em>&times;pGADT7-<em>BpSVP</em>1-6 were co-transferred into yeast Y2HGold. The yeast strains of Y2HGold［pGBKT7-<em>BpFLC</em>2-5&times;pGADT7-<em>BpSVP</em>］ could grew on selective agar plates TDO, QTO/A and QDO/X/A with blue stains. BpSVP truncated forms and BpFLC2-5 protein could act with each other to form heterodimers. Then, Y2HGold［pGBKT7-<em>BpFLC</em>&times;pGADT7-<em>BpSVP</em>2-5］ were brought into proximity to form protein compounds and activate transcription of four independent reporter genes of <em>AUR1</em>-<em>C</em>, <em>HIS</em>3, <em>ADE</em>2 and <em>MEL</em>1. The interactions between BpFLC3 and BpSVP3 were tested to confirm the acting domains. The yeast Y2HGold［pGBKT7-<em>BpFLC</em>3&times;pGADT7-<em>BpSVP</em>3］ exhibited blue stains on selective agar plates QDO/X/A. The K domain of BpFLC and that of BpSVP were the key structure domains and mediated the protein interactions between BpFLC and BpSVP.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2015.06.013