%0 Journal Article
%A 2
%A 2
%A 2
%A 2
%A 2
%A 2*
%A HU Jiao-Yang1
%A LUO Meng1
%A MU Fan-Song1
%A QIAO Qi1
%A SONG Zhuo-Yue1
%A ZU Yuan-Gang1
%T Optimization of Ultrasonic-assisted Extraction of Total Flavonoids from <em>Crataegus pinnatifida</em> Leaves and Its Antioxidant Activities
%D 2015
%R 10.7525/j.issn.1673-5102.2015.04.025
%J Bulletin of Botanical Research
%P 632-637
%V 35
%N 4
%X We used ultrasonic-assisted extraction(UAE) to extract the total flavonoids from <em>Crataegus pinnatifida</em> leaves, and used Box-Behnken design(BBD) combined with response surface methodology(RSM) to optimize extraction conditions. We determined the effects of four variables in terms of ratio of liquid/solid, temperature, ethanol concentration and time on the total flavonoids contents(TFC). The optimized extraction parameters obtained the highest total flavonoids yield were under the ratio of liquid/solid of 15, 40℃, and 40% of ethanol for 32 min. Under these conditions, the yield was 15.50 mg&middot;g<sup>-1</sup>. By in vitro antioxidant assays, the extract exhibited a strong DPPH radical-scavenging in ability with IC<sub>50</sub>&nbsp;value of 0.69 mg&middot;mL<sup>-1</sup>. Compared with conventional maceration extraction(ME) and heat-refluxing extraction(HE) methods, UAE method showed remarkable advantages in terms of better antioxidant activities. Overall, the present results indicated that UAE was a suitable method for the extraction of total flavonoids from <em>C.pinnatifida</em> leaves and its extracts were potential antioxidant ingredients.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2015.04.025