%0 Journal Article
%A 2
%A JIN Chu-Qi1
%A LIN Le-Jing1
%A ZHENG Chun-Hao3
%A ZHOU Ya-Mei1
%A ZHOU Ying1
%T ISSR Analysis on the Genetic Diversity of Relic Plant <em>Bretschneidera sinensis</em> from Wuyishan Region
%D 2015
%R 10.7525/j.issn.1673-5102.2015.04.014
%J Bulletin of Botanical Research
%P 559-563
%V 35
%N 4
%X <em>Bretschneidera sinensis</em> Hemsl., with important research value, is an endangered species endemic in East and South Chin. We used inter-simple sequence repeat(ISSR) markers studied the genetic diversity within and among six populations of <em>B.sinensis</em>. The genetic diversity at species level was high(<em>PPB</em>: 75.70%; <em>H</em><sub>ES</sub>: 0.304 5; <em>H</em>: 0.450 1). However, relatively low genetic diversity existed within populations. Population in Minjiangyuan(MJY) exhibited the greatest level of variability(<em>PPB</em>: 60.13%; <em>H</em><sub>EP</sub>: 0.238 1; <em>H</em>: 0.347 5), whereas the population in Baishanzu(BSZ) showed its own variability at the lowest level(<em>PPB</em>: 44.86%; <em>H</em><sub>E</sub>: 0.173 6; <em>H</em>: 0.255 6). By the genetic analysis of the ISSRs, we revealed a relatively high level of genetic differentiation among populations by Nei&rsquo;s gene diversity statistics(21.81%), Shannon&rsquo;s information measure(22.79%) and analysis of molecular variance(AMOVA)(18.62%). The main factors responsible for the relatively high level of differentiation among populations were probably related to the predominant outcrossing reproductive system and recent habitat fragmentation, and a significant correlation was between genetic distance and geographic distance(<em>r</em>=0.626 7, <em>P</em>&lt;0.05). The high diversity would hold promise for conservation and restoration efforts to save the endangered species from extinction.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2015.04.014