%0 Journal Article
%A 2
%A HU Feng1
%A HUANG Lie-Jian1*
%A SHI Qiong1
%T Tissue Culture System of Axillary Proliferation of <em>Acacia crassicarpa</em>
%D 2015
%R 10.7525/j.issn.1673-5102.2015.02.004
%J Bulletin of Botanical Research
%P 179-184
%V 35
%N 2
%X We studied the tissue culture system of <em>Acacia crassicarpa</em> by using the stemsegment with buds collected from 16-years plant as explants. The buds could be induced successfully on 2% of MS+sucrose with 67.33% of the survival rate and 80.56% of shooting rate after sterilized with 75% of alcoho1 and 0.1% of mercuric chloride for 30 s and 12 min, respectively. Low concentration of activated carbon effectively alleviated the verification, the best medium for multiplication was Modified-MS+6-BA 0.5 mg&middot;L<sup>-1</sup>+NAA 0.25 mg&middot;L<sup>-1</sup>+Ac 0.1 g&middot;L<sup>-1</sup>+sucrose 3% and the multiplication number was 3.72 after 35 days. The best medium for rooting was Modified-MS+IBA 0.5 mg&middot;L<sup>-1</sup>+2% of sucrose, and the rooting rate was 98.83%. The survival rate reached 90.00% after transplanting seedlings to the nutrition cup with yellow soil.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2015.02.004