%0 Journal Article
%A CAO Xiao-Ying
%A JIANG Ji-Hong*
%A LI Chang-Gen
%A LIU Qun
%A LU Fang
%A ZHENG Zhu-Jun
%T Molecular Cloning and Function Analysis of <em>HMGR</em> Gene from <em>Tilia miqueliana</em> Maxim
%D 2012
%R 10.7525/j.issn.1673-5102.2012.02.012
%J Bulletin of Botanical Research
%P 198-203
%V 32
%N 2
%X The 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is the key enzyme in the pathway for isoprenoid biosynthesis in plants. In this paper, a new HMGR gene, <em>TmiHMGR</em>, was first isolated from the leaves of <em>Tilia miqueliana</em> Maxim by rapid amplification of cDNA end technique. The full length cDNA of <em>TmiHMGR</em> was 2 160 bp, with a 1 758 bp open reading frame encoding a peptide of 585 amino acid residues. The deduced protein TmiHMGR has a calculated molecular weight of 62.9 kD and a pI value of 6.11. Phylogenetic tree analysis indicated that TmiHMGR had the closest relationship with HMGR from <em>Malusx domestica</em>. Tissue expression analysis revealed that <em>TmiHMGR</em> was strongly expressed in stems, while weakly in roots and leaves. The functional color complementation assay indicated that TmiHMGR could accelerate the biosynthesis of carotenoids in the <em>Escherichia coli</em> transformation, suggesting that TmiHMGR plays an influential role in isoprenoid biosynthesis.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2012.02.012