%0 Journal Article
%A AN Li-Zhe*
%A LIU Yu-Bing
%A SUN Kun
%A YANG Ning
%A ZHANG Teng-Guo
%T Cloning and Prokaryotic Expression of <em>Chorispora bungeana</em> CbMAPK3 Gene
%D 2009
%R 10.7525/j.issn.1673-5102.2009.06.009
%J Bulletin of Botanical Research
%P 692-695
%V 29
%N 6
%X Mitogen-activated protein kinase (MAPK) plays a central role in transfer information from diverse receptors/sensors to a wide range of cellular responses in plants. <em>CbMAPK</em>3 cDNA was amplified by RT-PCR from <em>Chorispora bungeana</em> leaf, then was cloned into pET-30a vector to construct recombinant prokaryotic expression vector pET-30a-CbMAPK3. The pET-30a-CbMAPK3 was transformed to <em>E.coli</em> strain of BL21. After induced by IPTG, a 46 kD recombinant protein was expressed and separated by SDS-PAGE electrophoresis and detected by western blot. The research provides a base for further studying on the protein structure and function of CbMAPK3.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2009.06.009