%0 Journal Article
%A WANG Zhi-Ying*
%A YU Wen-Jing
%A ZHANG Guo-Cai
%A ZOU Chuan-Shan
%T Populus nigra×Populus deltoids “108” Transformed the Chimeric Genes of Spider Insecticidal and Bt
%D 2009
%R 10.7525/j.issn.1673-5102.2009.05.017
%J Bulletin of Botanical Research
%P 603-606
%V 29
%N 5
%X Using the agrobacterium-mediated approach, the anti-insect gene (the chimeric genes of spider insecticidal and Bt) was successfully transformed into the acceptor of Populus nigra×Populus deltoids “108” as explant. Through PCR analysis, the agarose gel electrophoresis result displayed that six of eight Kanamycin fastness seedlings had target genes; Through PCRSouthern blots analysis, the result showed that the positive rate was 50%, and further indicated that the target genes had been inserted into the genomes of Populus nigra×Populus deltoids “108” plants successfully.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2009.05.017