%0 Journal Article
%A MA Chun-Hui
%A MENG Qing-Huan
%A WANG Hong-Zheng
%A YANG Lei*
%A ZHANG Xue-Ke
%A ZU Yuan-Gang
%T Simultaneous Determination of 4 Flavonoids in Extracts of <em>Phyllostachys pubescens</em> Leaves by HPLC
%D 2013
%R 10.7525/j.issn.1673-5102.2013.04.017
%J Bulletin of Botanical Research
%P 490-493
%V 33
%N 4
%X In this study, 4 flavonoids in <em>Phyllostachys pubescens</em> extracts i.e. orientin, vitexin, vitexin-4&prime;-O-glucoside, and vitexin-2&prime;-O-rhamnose glycoside were simultaneously detected. The HPLC detected conditions were as follows: Kromasil-C18 column (5 &mu;m, 4.6 mm&times;250 mm, Akzol Nobel, Sweden), mobile phase was tetrahydrofuran∶acetonitrile∶methanol∶0.5% aqueous acetic acid (10.3∶1∶0.7∶88), isocratic elution, the detection wavelength was 360 nm, column temperature was 25℃, and 1.0 mL&middot;min<sup>-1</sup> flow velocity. The average recoveries of orientin, vitexin, vitexin-4&prime;-O-glucoside, and vitexin-2&prime;-O-rhamnose glycoside were 98.5%, 98.1%, 99.1%, and 98.7%, respectively. The relative standard deviations were 0.8%, 0.7%, 0.8% and 0.6%(n=5), respectively. This HPLC method has been proved to be a method with high efficiency, rapid analysis, high sensitivity and good reproducibility for detection of flavonoids in <em>P.pubescens</em> extracts.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2013.04.017