%0 Journal Article
%A *
%A DUAN Ming-Hui
%A FU Yu-Jie
%A JIN Yan
%A YAO Xiao-Hui
%A ZHANG Dong-Yang
%T Aqueous Two-phase System(ATPS) Extraction of Hyperin
%D 2013
%R 10.7525/j.issn.1673-5102.2013.03.018
%J Bulletin of Botanical Research
%P 367-370
%V 33
%N 3
%X Based on the preliminary experiments, a three-variable, three-level Box-Behnken design was performed to study the aqueous two-phase system (ATPS) extraction of hyperin from <em>Pyrola calliantha</em> H. Andr.. The optimum parameters were as follows: extraction temperature 50℃, liquid /solid ratio 36∶1, (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> concentration 22%, extraction time 22 min, extraction power 300 W and three extraction cycles. Under the optimal conditions, the average extraction yield of hyperin was 0.982 mg&middot;g<sup>-1</sup> and the purity was 0.443%. This study is significant for the application of an aqueous two-phase system (ATPS) extraction technology and utilization of <em>P.calliantha</em> H. Andr..
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2013.03.018