Chromosome Preparation and Karyotype Analysis of Cordia subcordata

doi:10.7525/j.issn.1673-5102.2021.05.006

Abstract

Abstract:

In order to understand the genetic background of Cordia subcordata， we used the root tips as materials to investigate optimal sampling and pretreatment conditions， and the cells with better chromosome dispersion were selected for karyotype analysis. The results showed that：（1）The best sampling time of C. subcordata was from 9：30-10：00 am and from 14：00-14：30 pm and the optimal pretreatment was immersing the root tips in saturated p-dichlorobenzene for 2 hours；（2）C. subcordata was diploid， with 32 chromosomes， totally 16 pairs. The karyotype formula was 2n=2X=32=32m， and the karyotype was “1A”. The absolute length variation range of chromosome was 0.38-0.69 μm， and the relative length（%） varied from 4.48-8.24. The relative length composition was 2n=2L+14M1+14M2+2S. The karyotype asymmetry coefficient（As.K%） was 58.40%. These results would provide references for chromosome preparation and karyotype analysis， and laid a foundation for cytogenetic and epigenetic studies on C. subcordata.

Key words: Cordia subcordata, chromosome, karyotype analysis

CLC Number:

Q949.777.4

Yi-Lan CHEN, Hai-Min LIAO, Zheng-Feng WANG, Xiang-Xun HUANG, Dong-Ming LIU. Chromosome Preparation and Karyotype Analysis of Cordia subcordata[J]. Bulletin of Botanical Research, 2021, 41(5): 684-689.

Figures/Tables 7

Table 1

Fig.1

Table 2

Fig.2

Fig.3

Fig.4

Table 3

References 22

1	Bouby L，Bouchette A，Figueiral I.Sebesten fruits（Cordia myxa L.） in Gallia Narbonensis（Southern France）：a trade item from the Eastern Mediterranean？［J］.Vegetation History and Archaeobotany，2011，20（5）：397-404.
2	王文采.H.史密斯采集的中国紫草科植物［J］.植物研究，1993，13（1）：1-10.
	Wang W C.An enumeration of the boraginaceous plants collected by H.Smith from China［J］.Bulletin of Botanical Research，1993，13（1）：1-10.
3	刘东明，陈红锋，王发国，等.我国南沙群岛岛礁引种植物调查［J］.热带亚热带植物学报，2015，23（2）：167-175.
	Liu D M，Chen H F，Wang F G，et al.Investigation of introduced plants in Nansha Islands and reefs，China［J］.Journal of Tropical and Subtropical Botany，2015，23（2）：167-175.
4	吴淑华，陈昊雯，简曙光，等.中国热带珊瑚岛橙花破布木（Cordia subcordata）的生物学特性［J］.生态科学，2017，36（6）：57-63.
	Wu S H，Chen H W，Jian S G，et al.The biological characteristics of Cordia subcordataon tropical coral island in China ［J］.Ecological Science，2017，36（6）：57-63.
5	Matias E F F，Alves E F，Silva M K D N，et al.The genus Cordia：botanists，ethno，chemical and pharmacological aspects［J］.Revista Brasileira de Farmacognosia，2015，25（5）：542-552.
6	刘家熙，席以珍，宁建长，等.中国紫草科破布木属花粉形态和外壁超微结构［J］.植物学报，2001，43（9）：893-898.
	Liu J X，Xi Y Z，Ning J C，et al.Pollen morphology and exine ultrastructure of Genus Cordia in Boraginaceae in China［J］.Acta Botanica Sinica，2001，43（9）：893-898.
7	Abbott I A，Shimazu C.The geographic origin of the plants most commonly used for medicine by Hawaiians［J］.Journal of Ethnopharmacology，1985，14（2-3）：213-222.
8	陈蕙芳.治疗肝病的破布木属、番荔枝属和姜黄属植物提取物［J］.现代药物与临床，2010，25（1）：75.
	Chen H F.Extracts of Cordia，Annona and Curcuma for the treatment of liver diseases ［J］.Drugs & Clinic，2010，25（1）：75.
9	刘东明，简曙光，任海，等.一种橙花破布木扦插育苗的方法：中国，CN106900460A［P］.2017-06-30.
	Liu D M，Jian D S，Ren H，et al.A cuttage breeding method for ragwort of Cordia subcordata：China，CN106900460A ［P］.2017-06-30.
10	李懋学.植物染色体研究技术［M］.哈尔滨：东北林业大学出版社，1991：142-144.
	Li M X.The Chromosome Research Technology of Plants ［M］.Harbin：Northeast Forestry University Press，1991：142-144.
11	何丽娟.不同种类及种源枸杞染色体核型分析［D］.兰州：甘肃农业大学，2016.
	He L J.Chromosome karyotype analysis on different species and provenance of Lycium L.［D］.Lanzhou：Gansu Agricultural University，2016.
12	李懋学，陈瑞阳.关于植物核型分析的标准化问题［J］.武汉植物研究，1985，3（4）：297 -302.
	Li M X，Chen R Y.A suggestion on the standardization of karyotype analysis in plants［J］.Journal of Wuhan Botanical Research，1985，3（4）：297-302.
13	Levan A，Fredga K，Sandberg A A.Nomenclacture for centromeric position on chromosomes［J］.Hereditas，1964，52（2）：201-210.
14	Stebbins G L.Variantion and evolution in plants［M］.Co1umbia：Columbia University Press，1957.
15	雷文英，赵丽丽，简忠领，等.白刺花染色体压片技术优化及核型分析［J］.种子，2017，36（8）：5-9.
	Lei W Y，Zhao L L，Jian Z L，et al.Optimization of chromosome preparation and karyotype analysis of Sophora davidii［J］.Seed，2017，36（8）：5-9.
16	何倚涟，刘宏伟，杨彬，等.北柴胡根尖染色体制片技术研究［J］.种子，2018，37（8）：68 -71.
	He Y L，Liu H H，Yang B，et al.Study on chromosomes preparation technique of root tip of Bupleurum chinense DC［J］.Seed，2018，37（8）：68-71.
17	李国珍.染色体及其研究方法［M］.北京：科学出版社，1985.
	Li G Z.Chromosome and its research method［M］.Beijing：Science Press， 1985.
18	王丽平.黄杨属五种植物的核型分析［D］.南京：南京林业大学，2010.
	Wang L P.Karyotype analysis on five species of genus Buxus［D］.Nanjing：Nanjing Forestry University，2010.
19	杨牟.缙云山黄芩属（Scutellaria Linn.）植物的细胞学与叶表皮微形态分析［D］.重庆：西南大学，2015.
	Yang M.The Analysis of cytogenetic and the leaf epidermal microfeatures of Scutellaria Linn. in Jinyun mountain［D］.Chongqing：Southwest University，2015.
20	Gaviria J.Die Gattung Cordia in Venezuela［J］.Mitteilungen Der Botanischen Staatssammlung Munchen，1987，23：1-279.
21	Heubl G R，Gaviria J C，Wanner G.A contribution to the taxonomy and evolution of Cordia（Boraginaceae） and allied genera ［J］.Botanische Jahrbuecher fuer Systematik，1990，112：129-165.
22	Bhattacharya G N.Chromosome studies in Boraginaceae［J］.Bulletin of the Botanical Society of Bengal，1968，22：79-82.

序号 No.	绝对长度 Absolute length（μm）	相对长度Relative length（%）				相对长度系数 Coefficient of relative length	臂比 Arm ratio	着丝点位置 Centromere position
序号 No.	绝对长度 Absolute length（μm）	短臂 Short arm	长臂 Long arm	全长 Total length		相对长度系数 Coefficient of relative length	臂比 Arm ratio	着丝点位置 Centromere position
1	0.69	3.25	4.99	8.24		1.32（L）	1.53	m
2	0.65	3.10	4.57	7.67		1.23（M2）	1.48	m
3	0.62	3.05	4.32	7.38		1.18（M2）	1.42	m
4	0.61	3.06	4.12	7.19		1.15（M2）	1.34	m
5	0.59	2.92	4.04	6.96		1.11（M2）	1.38	m
6	0.57	2.64	4.10	6.73		1.08（M2）	1.55	m
7	0.55	2.55	3.99	6.54		1.05（M2）	1.56	m
8	0.54	2.70	3.73	6.43		1.03（M2）	1.38	m
9	0.52	2.53	3.68	6.21		0.99（M1）	1.46	m
10	0.50	2.46	3.44	5.90		0.94（M1）	1.40	m
11	0.48	2.28	3.39	5.67		0.91（M1）	1.48	m
12	0.46	2.41	3.08	5.49		0.88（M1）	1.28	m
13	0.44	2.33	2.91	5.24		0.84（M1）	1.25	m
14	0.43	2.27	2.78	5.05		0.81（M1）	1.23	m
15	0.41	2.02	2.80	4.82		0.77（M1）	1.39	m
16	0.38	2.02	2.46	4.48		0.72（S）	1.22	m
核型类型：1A					核型公式：2n=2X=32=32m

[1]	Xiayu HU, Yuping LIU, Xu SU, Ping YANG, Yanan WANG. Chromosome Number and Karyotype Analysis of Different Populations in Sophora alopecuroides (Fabaceae) [J]. Bulletin of Botanical Research, 2023, 43(1): 9-19.
[2]	Jiawei XIAO, Yingjia WANG, Wenxuan HUANG, Weiping LI, Gongping KANG. Supplementary Description on Morphology and Cytology of Aster longipetiolatus C.C.Chang (Asteraceae) [J]. Bulletin of Botanical Research, 2022, 42(5): 741-745.
[3]	DING Hai-Yan. Fast Manufacture of Meiotic Chromosome Specimens of the Plant Material [J]. Bulletin of Botanical Research, 2017, 37(5): 797-800.
[4]	YANG Liu-Hui, ZHANG Jian-Jun, WANG Qi, ZHU Wei, ZHANG Teng, YU Xiao-Nan. Ploidy Identification and Karyotype Analysis of Five Itoh Hybrid Peonies [J]. Bulletin of Botanical Research, 2017, 37(4): 535-541.
[5]	LI Yun-Ling, TIAN Bao-Ming, YANG Yan, CUI Ming-Zhu, HAO Zhi-Da, WEI Fang. Molecular and Cytological analysis of Meiosis in Autotetraploid Arabidopsis thaliana [J]. Bulletin of Botanical Research, 2016, 36(3): 354-359.
[6]	HE Yang-Yan;MA Dan-Wei;LI Qun;WANG Ya-Nan;TIAN Jia-Qiao;CHENG Wei-Wei. Temperature Influences on the Allelopathy Effect of Aqueous Extracts from Trifolium repens* L. [J]. Bulletin of Botanical Research, 2010, 30(2): 243-247.
[7]	WU Jian-Bo;CHEN Cheng-Bin;BAO Xiao-Ying;SONG Wen-Qin;ZHAO Nian-Xi;GAO Yu-Bao. Chromosome Numbers and Karyotypes of Stipa baicalensis,Stipa grandis* and Stipa krylovii in Inner-mongolia Steppe [J]. Bulletin of Botanical Research, 2009, 29(5): 534-538.
[8]	CHANG Zhao-Yang;LI Bin;SHI Fu-Chen;. The Chromosomes and Karyotypes of Some Species in Caragana from China [J]. Bulletin of Botanical Research, 2009, 29(1): 18-24.
[9]	HU Feng-Rong;LIU Guang-Xin;XI Meng-Li;WU Zhu-Hua;SHI Ji-Sen. Chromosome Giemsa C-banding Analysis of Lilium leucanthum*(Baker) Baker [J]. Bulletin of Botanical Research, 2009, 29(1): 12-14.
[10]	CHEN Xia;XING Lu-Lu;DING Hai-Yan;XU Ying-Bo;LI Ji-Lin*. Study on Meiosis Comparison of Pollen Grain of F₁ Hybrided by Gametocidal Substitution Lines and Wheat [J]. Bulletin of Botanical Research, 2008, 28(4): 392-395.
[11]	FAN Bao-Li;LI Chun-Zheng;PENG Yong-Kang. Comparison of the Protein Composition and Analysis of the Specific Protein from Wheat-Haynaldid villosa* Chromosome Substitution Line and Translocation Line by 2D-PAGE [J]. Bulletin of Botanical Research, 2007, 27(3): 297-301.
[12]	LIU Feng-Qi;QU Min;ZHANG Yan-Ming;ZHANG Xue-Ting;LI Ji-Lin*. Cytogenetic Studies on the Cross-progenies Between Chinese Spring-Agilops 2C Disomic Addition and Chinese Spring-Elytriga Disomic Addition [J]. Bulletin of Botanical Research, 2007, 27(1): 89-93.
[13]	KONG Hong. Karyotypes of three species in Glycyrrhiza [J]. Bulletin of Botanical Research, 2006, 26(5): 560-562.
[14]	CAO Tong, SHA Wei, SUN Jun, WANG Yan-jun. A CYTOLOGICAL OBSERVATION ON 6 LIVERWORTS SPECIES FROM NORTHEAST CHINA [J]. Bulletin of Botanical Research, 2002, 22(4): 453-455.
[15]	SUN Zhong-ping, WANG Zhan-bin, XU Xiang-ling, LI Ji-lin. THE EFFECT OF GAMETOCIDAL CHROMOSOME 2C DERIVED FROM AEGILOPS CYLINDRYCA ON MEIOSIS AND MITOSIS OF POLLEN GRAINS OF TRITICALE [J]. Bulletin of Botanical Research, 2002, 22(4): 479-482.