Fig.1

Seedlings of Lilium davidii var. unicolor after different sub-cultured days

Table 1

Single-factor experimental parameters of protoplast isolation and purification of Lilium davidii var. unicolor

Fig.2

Effect of plant materials sub-cultured for different days on protoplast isolation yield and viability of Lilium davidii var. unicolorDifferent lowercase letters indicated statistical differences，the same as below.

Fig.3

Effect of mannitol concentration on protoplast isolation yield and viability of Lilium davidii var. unicolor

Fig.4

Effect of cellulase and macerozyme concentrations on protoplast isolation yield and viability of Lilium davidii var. unicolor

Fig5

Effect of enzymatic hydrolysis time on protoplast isolation yield and viability of Lilium davidii var. unicolor

Fig.6

Effect of sucrose mass concentration on protoplast purification of Lilium davidii var. unicolorA.The effect of purification with different sucrose mass concentration on protoplast yield and viability（Different lowercase letters indicated statistical differences）；B.Protoplasts before purification；C-F.Effect of purification by 200-280 g·L-1 sucrose solution on protoplast number and impurities.

Fig.7

Expression of green fluorescent protein（GFP） in the protoplasts of Lilium davidii var. unicolor

Fig.8

Protoplast fusion of Lilium davidii var. unicolorA.Protoplast suspension before dilution；B.Protoplast suspension after dilution， the protoplasts were round-shaped and did not aggregate；C.The aggregation of multiple cells during the fusion reaction；D.Two cells began to contact；E.The two cells began to fuse，and the nucleus at the white arrow was gourd-shaped；F.The fusion of the two cells was completed，and the hybrid cell gradually returned to spherical shape.