Construction of Suppression Expression Vector and Genetic Transformation of BpSPL9 gene from Betula platyphylla

doi:10.7525/j.issn.1673-5102.2023.01.004

Abstract

Abstract:

To clarify the function of SPL gene in growth and development of Betula platyphylla，the BpSPL9 gene was cloned， and the expression vector of BpSPL9 was constructed by CREST technology， and the genetic transformation was carried out by Agrobacterium mediated method， and the phenotype of the transgenic lines was observed， respectively. The results showed that the transgenic lines with BpSPL9 suppressed were successfully obtained. The height of transgenic lines was significantly lower than that of non-transgenic lines， with shorter node spacing and smaller leaf area. The contents of 6-BA and IAA in transgenic lines were lower than those of the non-transgenic lines. It is speculated that BpSPL9 gene participated in plant growth and development by affecting the synthesis of auxin and cell division.

Key words: Betula platyphylla, SPL9, CREST, genetic transformation

CLC Number:

S792.153

Jinxia DU, Tingting SHEN, Haoran WANG, Yiping LIN, Huiyu LI, Lianfei ZHANG. Construction of Suppression Expression Vector and Genetic Transformation of BpSPL9 gene from Betula platyphylla[J]. Bulletin of Botanical Research, 2023, 43(1): 30-35.

Figures/Tables 8

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