Effects of Sucrose on Growth and Physiological Characteristics in vitro Plantlets of 84K Poplar

doi:10.7525/j.issn.1673-5102.2021.01.010

Abstract

Abstract:

Carbon source has an important effect on the growth and quality in vitro plantlets. The effects of different sucrose concentrations（10，20，30 and 40 g·L^-1） on the growth and physiological characteristics in vitro plantlets of 84K poplar were studied. The results showed that with the 30 g·L^-1 sucrose was the most favorable for the growth in vitro plantlets of 84K poplar with the culture period increased，and the dry weight was 1.5 times that of 10 g·L^-1 sucrose concentration treatment. Secondly，in the treatment of higher sucrose concentration，with the culture period increased，the in vitro plantlets of 84K poplar had higher chlorophyll content，Rubisco enzyme activity and net photosynthetic rate，of which 30 g·L^-1 sucrose treatment in vitro plantlets of 84K poplar had the highest net photosynthetic rate，which was 3.617 μmol?m^-2?s^-1. The in vitro plantlets of 84K poplar treated with high concentration of sucrose had higher soluble sugar content，but no significant change in starch content. To sum up，sucrose concentration has an important influence on the growth and physiological characteristics in vitro plantlets of 84K poplar. Higher sucrose concentration was conducive to promoting the growth in vitro plantlets of 84K poplar.

Key words: sucrose, tissue culture regenerated plantlets, growth, physiological characteristics

CLC Number:

S792.11

Sen SHI, Na MIAO, Yu-Tong SHI, Hui-Mei WANG. Effects of Sucrose on Growth and Physiological Characteristics in vitro Plantlets of 84K Poplar[J]. Bulletin of Botanical Research, 2021, 41(1): 74-78.

Figures/Tables 6

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