AtAAPT1基因RNAi的植物表达载体的构建

doi:10.7525/j.issn.1673-5102.2011.03.011

植物研究 ›› 2011, Vol. 31 ›› Issue (3): 313-317.doi: 10.7525/j.issn.1673-5102.2011.03.011

AtAAPT1基因RNAi的植物表达载体的构建

郑桂灵;李鹏

西南科技大学生命科学与工程学院，绵阳　621010

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-05-20 发布日期:2011-05-20
基金资助:

Construction of Plant Transformation Vector of AtAAPT1 Gene for RNAi

ZHENG Gui-Ling;LI Peng

School of Life Sciences and Engineering,Southwest University of Science and Technology,Mianyang　621000

Received:1900-01-01 Revised:1900-01-01 Online:2011-05-20 Published:2011-05-20
Supported by:

摘要/Abstract

摘要： 以拟南芥中氨基乙醇磷酸转移酶基因AAPT1作为RNAi的靶向序列，采用RT-PCR的方法获得目的DNA片段。然后以pBS-T-AAPT1载体为基础,构建了由35s启动子调控的AAPT1基因RNAi的植物表达载体pART27-AAPT1(1,2),并通过电击法将重组质粒导入根癌农杆菌C58中。AtAAPT1基因RNAi的植物表达载体的构建对于研究AAPT1基因的功能和应用具有重要的理论价值。

关键词: AAPT1基因, RNAi, 植物表达载体

Abstract: Aminoalcoholphosphotransferase gene AAPT1 from Arabidopsis thaliana was used as target sequence for RNA interference in this paper. The target sequence was linked to pBS-T cloning vector after obtained by RT-PCR reaction. A plant transformation vector pART27-AAPT1(1,2) was constructed from pBS-T, including AAPT1 gene controlled by CaMV35s promoter in this experiment. Then the recombination plasmid was transfected into Agrobacterium C58 by electroporation-mediated way.

Key words: AAPT1 gene, RNAi, plant transformation vector

中图分类号:

Q291

郑桂灵;李鹏. AtAAPT1基因RNAi的植物表达载体的构建[J]. 植物研究, 2011, 31(3): 313-317.

ZHENG Gui-Ling;LI Peng. Construction of Plant Transformation Vector of AtAAPT1 Gene for RNAi[J]. Bulletin of Botanical Research, 2011, 31(3): 313-317.

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AtAAPT1基因RNAi的植物表达载体的构建

Construction of Plant Transformation Vector of AtAAPT1 Gene for RNAi

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